Figure 1.

Media formulation of RPMI, DMEM, and HPLM. (a) All experiments were performed in these media supplemented by 10% dialyzed FBS. Glucose concentration of DMEM and RPMI was modified as physiological concentration (low glucose, 5 mM). (b) The chart shows the relative concentrations of RPMI and DMEM media supplemented with 5 mM glucose compared with HPLM. Components not present in RPMI and DMEM but present in HPLM are denoted as “absent”; components not present in the HPLM but present in the other media are denoted as “present”.