Figure 5.

Interactions of the two breast adipose progenitor cell (APC) populations with MCF7 mammospheres. Time-lapse analysis of breast cancer mammospheres spreading on Populations 1 (A–D) and 2 (E–H) was performed on cells differentiated for 21 days. They were incubated for 24 h with MCF7 mammospheres expressing GFP prior to recording for the mammospheres to adhere to the cells. Recording lasted for 63 h and was performed every 20 min, using an inverted AxioObserver-Zeiss microscope equipped with a sCMOS ANDOR Neo camera at 37 °C in a CO2-controlled atmosphere (magnification 10×). The area covered by the mammospheres was analyzed by using IMAGE J. The red image delineates the area after defining the threshold level to ignore non-specific signals. The increase in mammosphere size was calculated by the ratio of the areas measured at the beginning and at the end of the experiment. (I) Box plots of the data are presented for each patient in Supplementary Table S2 (** p < 0.01).