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. 2022 Aug 10;12(8):1101. doi: 10.3390/biom12081101

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Comparison of ganglia from the proximal normoganglionic tissue in non-HAEC and HAEC patients. (A,B) Representative images of ganglia from the patient groups, with immunohistochemistry performed against DAPI, HuC/D and S100B. Note: (C) Quantification of the muscle unit to ganglia ratio (ns: p > 0.05, Students’ t-test). (D) Quantification of the size of ganglia (#DAPI+ cells/ganglion, left), the proportion of neurons per ganglion (%Hu + /DAPI+, middle), and the ratio of glia/neurons per ganglion (right). non-HAEC: n = 21 ganglia, N = 4 patients; HAEC: n = 21 ganglia, N = 3 patients. * p = 0.0158, ns: p > 0.05, Students’ t-test.