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. 2022 Aug 12;12(8):1111. doi: 10.3390/biom12081111

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SDS-PAGE analysis in non-reducing (A) and reducing (B) conditions of the consecutive SEC-MALS oligomer fractions with the elution time range of 22–32 min. An amount of 10 µg was loaded into each well. The average mass distribution over the analyzed fractions in the non-reducing conditions is inversely proportional to the SEC-MALS elution time, as expected. The higher the molecular weights, the more unstable the cytotoxin. The oligomeric fractions additionally contain monomer, dimer, and aggregates. The efficient oligomers monomerization was observed in the reducing conditions.