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. 2022 Aug 16;10(8):1986. doi: 10.3390/biomedicines10081986

Table 1.

Primer sequences and annealing temperatures for the measured genes.

Forward Primer Reverse Primer Annealing Temperature
NF-κb 5′-CAATGGCTACACAGGACCA-3′ 5′-CACTGTCACCTGGAACCAGA-3′ 52 °C
TNF-α 5′-TCTACTGAACTTCGGGGTGATCG-3′ 5′-TGATCTGAGTGTGAGGGTCTGGG-3′ 56 °C
p53 5′-ACCGCCGACCTATCCTTACC-3′ 5′-TCTTCTGTACGGCGGTCTCTC-3′ 56 °C
β-actin 5′-ACGGCCAGGTCATCACTATTG-3′ 5′-CAAGAAGGAAGGCTGGAAAAGA-3′ 52 °C
miR-29a 5′-GCGCACTGATTTCTTTTGGTGTTCAG-3′ 5′-GCGAGCACAGAATTAATACGAC-3′ 51 °C
RNU6B 5′-CTCGCTTCGGCAGCACATA-3′ 5′-CGCTTCACGAATTTGCGTG-3′ 53 °C

NF-κB: nuclear factor kappa B; TNF-α: tumor necrosis factor alpha; RNU6B: U6B small nuclear RNA.