Table 1.
Forward Primer | Reverse Primer | Annealing Temperature | |
---|---|---|---|
NF-κb | 5′-CAATGGCTACACAGGACCA-3′ | 5′-CACTGTCACCTGGAACCAGA-3′ | 52 °C |
TNF-α | 5′-TCTACTGAACTTCGGGGTGATCG-3′ | 5′-TGATCTGAGTGTGAGGGTCTGGG-3′ | 56 °C |
p53 | 5′-ACCGCCGACCTATCCTTACC-3′ | 5′-TCTTCTGTACGGCGGTCTCTC-3′ | 56 °C |
β-actin | 5′-ACGGCCAGGTCATCACTATTG-3′ | 5′-CAAGAAGGAAGGCTGGAAAAGA-3′ | 52 °C |
miR-29a | 5′-GCGCACTGATTTCTTTTGGTGTTCAG-3′ | 5′-GCGAGCACAGAATTAATACGAC-3′ | 51 °C |
RNU6B | 5′-CTCGCTTCGGCAGCACATA-3′ | 5′-CGCTTCACGAATTTGCGTG-3′ | 53 °C |
NF-κB: nuclear factor kappa B; TNF-α: tumor necrosis factor alpha; RNU6B: U6B small nuclear RNA.