Table 1.

A list of membrane solubilizing detergents commonly used for membrane protein isolation.

(1) Ionic Detergents

Sodium dodecyl sulfate (SDS; anionic)

Deoxycholate (anionic; bile acid salt)

Sodium cholate (anionic; bile acid salt)

Calixarene (anionic)

N-lauryl sarcosine or sarkosyl (anionic)

Cetyltrimethylammonium bromide (CTAB; cationic)

Hexadecyltrimethylammonium bromide (cationic)

(2) Zwitterionic detergents

3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)

3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO)

n-dodecyl-N, N-dimethylamine-N-oxide (LDAO)

n-dodecyl phosphocholine (DPC)

(3) Nonionic detergents a

Poly-oxyethyleneglycol lauryl ether

n-dodecyl-β-D-maltoside (DDM)

n-nonyl-β-D-glucoside (NG)

n-octylglucoside (OG)

Polyethylene glycol tert-octyl phenyl ether (Triton X-100)

Undecyl-β-D-maltoside (UDM)

Digitonin

Maltose neopentyl glycol (MNG)

Hecameg [6-O-(N-heptylcarbamoyl)-methyl-α-D-glucopyranoside (HG)

^a Long-chain (C₁₂–C₁₄) nonionic detergents are milder than short-chain (C₇–C₁₀) nonionic detergents.