Table 4.
List of membrane proteins isolated using polymers under detergent-free conditions.
| Prokaryotic Protein | Eukaryotic Protein |
|---|---|
| SMA (Anionic) | |
| Listeria NDH-2a enzyme from the pathogenic bacteria Listeria monocytogenes, expressed in E. coli [60] | GPCR [human adenosine A2A receptor (A2AR)] expressed in P. pastoris and human embryonic kidney (HEK)293T cells [69] |
| E. coli rhomboid protease GlpG (with a six α-helix transmembrane domain) expressed in E. coli [97] | hENT1 expressed in Sf9 insect cells [98] |
| Bacterial divisome, single transmembrane protein ZipA [110] | Protein complex (CytcO along with Rcf subunits from S. cerevisiae subcellular membranes such as mitochondria) [113] |
| K+ importer A (KimA) from Bacillus subtilis and sodium–solute symporter protein (SSS) expressed in E.coli [137] | Wild-type human GPCR; dopamine receptor 1 (D1) expressed in HEK293f cells [115] |
| KcsA; it is a tetrameric potassium channel from Streptomyces lividans expressed in E. coli [117] | Human tetraspanins (four helical transmembrane domains) [116] |
| E. coli trimeric multidrug efflux transporter AcrB expressed in E. coli [156] | Protein complex from Saccharomyces cerevisiae subcellular membranes such as mitochondria [130] |
| Potassium importer KimA from Bacillus subtilis expressed in E. coli [157] | Human Pgp (P-glycoprotein; ABCB1) expressed in High Five (Trichoplusia ni) insect cells [140] |
| α-helical seven-transmembrane proton pump bacteriorhodopsin from Haloquadratum walsbyi (HwBR) expressed in E. coli [119] | Plant plasma membrane Na+/H+ antiporter SOS1 (Salt Overlay Sensitive 1) of Arabidopsis thaliana expressed in P. pastoris [158] |
| Holo-translocon (HTL)—a supercomplex of SecYEG–SecDF–YajC–YidC proteins [159] | RhD antigen from RBCs [160] |
| 2×34 kDa cation diffusion facilitator protein from Cupriavidus metallidurans CH34 for proton-detected solid-state NMR [161] | A2αR and CGRP (GPCRs) receptors expressed in P. pastoris and Cos7 cells, respectively [162] |
| PglC and PglA of Campylobacter jejuni expressed in E. coli [163] | P-glycoprotein (ABC transporter family) isolated from MCF-7/ADR cells for small molecule screening by SPR [148] |
| The membrane tether protein ZipA and the ATP binding cassette (ABC) transporter BmrA are expressed in E. coli [162] | Human multidrug resistance protein 4/ABCC4 (MRP4/ABCC4) (expressed in Sf9 cell-lines) [141] |
| Penicillin-binding protein complex PBP2/PBP2a from Staphylococcus aureus [164] | Human adenosine 2α receptor (A2αR; GPCR) expressed in Pichia pastoris [149] |
| (a) BmrA from Bacillus subtilis (a homodimer; each monomer provides six transmembrane α-helices and a cytosolic nucleotide-binding domain, (b) LeuT from Aquifex aeolicus (an amino acid:sodium symporter; comprising 12 transmembrane helices; and (c) ZipA from E. coli (a single transmembrane helix with a large cytosolic domain) [165] | (a) Full-length pore-forming α-subunits hKCNH5 and hKCNQ1 of human neuronal and cardiac voltage-gated potassium (KV) channels, (b) the fusion protein comprising of an α-subunit hKCNQ1 and its regulatory transmembrane KCNE1 β-subunit (hKCNE1-hKCNQ1), expressed in mammalian COS-1 cells [150] |
| E. coli tyrosine kinase with two transmembrane helices for 19F-NMR [166] | Dynamic dhurrin metabolon from the microsomes of Sorghum bicolor [167], photosystem I light-harvesting chlorophyll II supercomplex from spinach [168] |
| SecYEG in complex with SecA from E. coli [169] | Human ATP binding cassette ABCG2 is expressed in HEK293T cells [145] |
| AcrB from Salmonella typhimurium [170] | Melatonin MT1R (GPCR) [144] expressed in P. pastoris |
| E. coli cytochrome bo3; expressed in E. coli [171] | Human tetraspanins CD81; expressed in P. pastoris [155] |
| Bacterial pLGIC; expressed in E. coli [172] | Slow anion channel 1 (SLAC1) from Brachypodium distachyon, its structure is determined using cryo-EM at 2.97 Å [173], expressed in S. pombe |
| Thermally stable rhodopsin from Rubrobacter xylanophilus rhodopsin (RxR) and an unstable one from Halobacterium salinarum sensory rhodopsin I [174]; expressed in E. coli cells BL21 (DE3) | Rhodopsin, ABCA4, and PRPH2/ROM1 from mice [135] |
| SARS-CoV-2 S glycoprotein expressed in 293T cells | Renal outer medullary potassium channels (ROMK) are expressed in E. coli [128] |
| Mycobacterial membrane protein large 3 (Mmpl3) from Mycobacterium tuberculosis [175] | Human β2-adrenergic receptor expressed in HEK293T cell line [112] |
| BAM-MBP-76EspP co-complex; expressed in E. coli BL21 (DE3) [136] | Trimeric photosystem I from the cyanobacterium Thermosynechococcus elongates [176]. |
| Sav1866 (ABC transporter) from Staphylococcus aureus, expressed in E. coli BL21 [177] | Spinach and pea thylakoid membrane protein complexes [151] |
| KcsA expressed in E. coli BL21(λDE3), isolated suing SMA analogues [101] | Yeast transmembrane sensor Wsc1 [152] |
| Succinate dehydrogenase (Sdh4) [153] | |
| Arabidopsis thaliana cytochrome-b5 expressed in E. coli [178] | |
| BAK protein from mitochondria of BAK KO U2OS cells [179] | |
| Smoothened (SMO) GPCR protein expressed Sf9 insect cells [146] | |
| DIBMA (anionic) | |
| The membrane tether protein ZipA and the ATP Binding Cassette (ABC) transporter BmrA [162]; expressed in E. coli | GPCRs: A2αR and CGRP receptor; expressed in P. pastoris and Cos7 cells, respectively [162] |
| Bacterial OmpLA [132]; expressed in E. coli BL21 (DE3) | Human serotonin transporter (hSERT); expressed in Pichia pastoris [142] |
| E. coli rhomboid protease GlpG and Vibrio cholerae rhomboid protease [127] | |
| E. coli ZipA [180] | |
| Glyco-DIBMA (anionic) | |
| Voltage-gated K+ channel KvAP; expressed in E. coli [107] | |
| SMA-QA (cationic) and SMA-EA (anionic) | |
| Rabbit cytochrome b5; expressed in E. coli [55] | |
| Polymethacrylate (PMA) | |
| Neurotensin type 1 receptor; expressed in Sf9 cells [181] | |
| Pentyl-inulin (Non-ionic) | |
| FBD domain of CYP450-reductase; expressed in E.coli [58] | |