Figure 3.

ITC characterization of the interactions of TgCEN1 and its domains with the R17 and R12 peptides in the presence of CaCl₂ or EGTA. Representative thermograms (top panels) and the derived binding isotherms (bottom panels) of titration of R17 (A) and R12 (B) into TgCEN1, N-TgCEN1, and C-TgCEN1 in the presence of 5 mM CaCl₂ or 5 mM EGTA at 25 °C. The ligand dilution blank experiments (peptide titrated into buffer) were subtracted from the binding isotherm obtained in the presence of protein. A first injection of 0.2 μL was made and then the first data point was removed from data fitting.