Table 1.
Summary of the thermodynamic parameters of the interaction between TgCEN1 and its N-terminal and C-terminal domains with the peptides derived from TgSFI1. n—stoichiometry of binding; Kd—dissociation constant; T—298 K.
| Buffer | n | Kd (µM) |
ΔH (kcal mol−1) |
TΔS (kcal mol−1) |
|
|---|---|---|---|---|---|
| TgCEN1 + R10 | CaCl2 | n1 = 0.7 ± 0.1 | 0.014 ± 0.002 | −19.1 ± 0.4 | −6.3 ± 0.4 |
| n2 = 0.9 ± 0.1 | 2.5 ± 0.3 | −12.1 ± 0.3 | −4.4 ± 0.3 | ||
| EGTA | 0.8 ± 0.1 | 0.8 ± 0.1 | −24.7 ± 0.4 | −16.4 ± 0.4 | |
| N-TgCEN1 + R10 | CaCl2 | 0.7 ± 0.1 | 1.2 ± 0.4 | −12.6 ± 0.9 | −4.4 ± 1.0 |
| EGTA | - | - | - | - | |
| C-TgCEN1 + R10 | CaCl2 | 0.7 ± 0.1 | 0.027 ± 0.005 | −15.8 ± 0.1 | −5.5 ± 0.5 |
| EGTA | 0.7 ± 0.1 | 1.8 ± 0.1 | −19.3 ± 0.1 | −11.5 ± 0.7 | |
| TgCEN1 + R16 | CaCl2 | n1 = 0.7 ± 0.1 | 0.009 ± 0.001 | −20.9 ± 2.5 | −9.9 ± 2.4 |
| n2 = 0.8 ± 0.1 | 0.5 ± 0.1 | −11.8 ± 2.7 | −3.3 ± 1.7 | ||
| EGTA | 0.9 ± 0.2 | 3.4 ± 0.3 | −26.2 ± 3.4 | −18.7 ± 3.3 | |
| N-TgCEN1 + R16 | CaCl2 | 0.7 ± 0.1 | 0.4 ± 0.1 | −11.6 ± 0.6 | −2.8 ± 0.7 |
| EGTA | - | - | - | - | |
| C-TgCEN1 + R16 | CaCl2 | 0.7 ± 0.1 | 0.006 ± 0.001 | −18.8 ± 1.5 | −8.9 ± 1.6 |
| EGTA | 0.8 ± 0.1 | 9.1 ± 0.8 | −22.4 ± 0.6 | −15.7 ± 2.2 | |
| TgCEN1 + R17 | CaCl2 | 0.7 ± 0.1 | 0.8 ± 0.2 | −18.1 ± 0.5 | −9.7 ± 0.7 |
| EGTA | - | - | - | - | |
| N-TgCEN1 + R17 | CaCl2 | - | - | - | - |
| EGTA | - | - | - | - | |
| C-TgCEN1 + R17 | CaCl2 | 0.9 ± 0.1 | 2.7 ± 0.1 | −16.9 ± 0.2 | −9.3 ± 0.3 |
| EGTA | - | - | - | - | |
| TgCEN1 + R24 | CaCl2 | 0.9 ± 0.1 | 3.4 ± 0.6 | −8.9 ± 0.9 | −2.1 ± 0.7 |
| EGTA | - | - | - | - | |
| N-TgCEN1 + R24 | CaCl2 | - | - | - | - |
| EGTA | - | - | - | - | |
| C-TgCEN1 + R24 | CaCl2 | 0.8 ± 0.1 | 2.6 ± 0.3 | −8.6 ± 0.2 | −0.9 ± 0.3 |
| EGTA | - | - | - | - | |
| TgCEN1 + R31 | CaCl2 | 0.8 ± 0.1 | 2.9 ± 0.1 | −24.2 ± 1.4 | −16.6 ± 1.5 |
| EGTA | - | - | - | - | |
| N-TgCEN1 + R31 | CaCl2 | - | - | - | - |
| EGTA | - | - | - | - | |
| C-TgCEN1 + R31 | CaCl2 | 0.8 ± 0.1 | 1.9 ± 0.1 | −22.2 ± 0.3 | −14.4 ± 0.3 |
| EGTA | - | - | - | - | |
| TgCEN1 + R5 | CaCl2 | 0.7 ± 0.1 | 0.5 ± 0.1 | −26.4 ± 2.4 | −22.8 ± 1.8 |
| EGTA | 0.6 ± 0.1 | 36.9 ± 6.6 | −24.2 ± 2.1 | −10.6 ± 2.3 | |
| N-TgCEN1 + R5 | CaCl2 | - | - | - | - |
| EGTA | - | - | - | - | |
| C-TgCEN1 + R5 | CaCl2 | 0.7 ±0.1 | 0.9 ± 0.1 | −23.3 ± 0.1 | −15.1 ± 0.3 |
| EGTA | 0.9 ± 0.3 | 47.8 ± 4.9 | −10.2 ± 3.1 | −14.4 ± 3.2 | |
| TgCEN1 + R12 | CaCl2 | 0.9 ± 0.1 | 0.5 ± 0.1 | −21.5 ± 1.3 | −12.7 ± 1.2 |
| EGTA | 1.1 ± 0.1 | 29.8 ± 3.4 | −20.4 ± 2.4 | −14.2 ± 2.5 | |
| N-TgCEN1 + R12 | CaCl2 | - | - | - | - |
| EGTA | - | - | - | - | |
| C-TgCEN1 + R12 | CaCl2 | 0.8 ± 0.1 | 0.4 ± 0.1 | −18.5 ± 0.1 | −9.8 ± 0.5 |
| EGTA | 1.2 ± 0.5 | 69.9 ± 7.0 | −13.3 ± 1.9 | −7.6 ± 2.9 |