Figure 2.

The manipulation of FAK expression regulates the functional roles of EZH2. (A) A549 or H1299 cells overexpressing GFP or GFP–FAK were lysed and subjected to Western blot analysis. The protein levels were detected using the indicated antibodies. A slight increase in EZH2 levels was detected in cells overexpressing GFP–FAK. (B) H1299 cells transfected with shFAK for FAK depletion were lysed and subjected to Western blot analysis. Protein levels were detected using the indicated antibodies. As a loading control, an anti-β-actin antibody was used. Western blot analysis revealed that FAK depletion decreased EZH2 expression and reduced downstream signaling, based on the assessment of the tri-methylation at Lys9 and Lys27 on histone H3.