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. 2022 Aug 10;10(8):1937. doi: 10.3390/biomedicines10081937

Figure 4.

Figure 4

EZH2 knockdown induces cellular senescence in non-small cell lung cancer cells. (A) A549 or H1299 cells were transfected with shRNA to deplete EZH2 and incubated for 4 days. SA-β-Gal staining was employed to measure the percentage of senescent cells (β-gal-positive cells). The bar chart shows that <3% of cells in the shLuc population were SA-β-gal-positive, whereas more than 10% of the shFAK cells population were SA-β-gal positive, based on three independent experiments (n > 250). *** p < 0.001 according to a Student’s t-test. (B) A549 or H1299 cells were treated with shRNA to deplete EZH2, lysed, and subjected to Western blot analysis. Protein levels were detected using the indicated antibodies. As a loading control, an anti-β-actin antibody was used. The numbers below the blot images indicate the relative intensity of EZH2 or lamin A/C signal relative to the β-actin signal.