TABLE 3.
The 24 metabolites associated with PLA2G6 mutation.
| Compound | Canonical pathway | Mann–Whitney U-testing |
t-test after FDR (q value) |
Binary logistic regression | ROC in PLA2G6 mutation |
ROC in advanced stage |
|||
| AUC | P-value | AUC | P-value | ||||||
| 1 | 1-Hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1′-rac-glycerol) | 0.021 | 0.044 | 0.067 | 0.833 | 0.021 | 0.606 | ||
| 2 | 1-Hexadecanoyl-sn-glycero-3-phosphoethanolamine | 0.009 | 0.015 | 0.06 | 0.875 | 0.009 | 0.439 | ||
| 3 | 1-Oleoyl-L-alpha-lysophosphatidic acid | 0.005 | 0.016 | 0.04* | 0.903 | 0.005 | 0.944 | 0.039 | |
| 4 | Caprylic acid | Fatty acid biosynthesis | 0.003 | 0.018 | 0.061 | 0.931 | 0.003 | 1 | |
| 5 | L-Palmitoylcarnitine | Fatty acid degradation | 0.007 | 0.015 | 0.038* | 0.889 | 0.007 | 0.606 | |
| 6 | Oleic acid | Biosynthesis of unsaturated fatty acids | 0.001 | 0.010 | 0.992 | 1 | 0.001 | 0.944 | 0.039 |
| 7 | PC(16:0/16:0) | Arachidonic acid metabolism | 0.034 | 0.029 | 0.083 | 0.806 | 0.034 | 0.439 | |
| 8 | Glycochenodeoxycholate | Primary bile acid biosynthesis | 0.009 | 0.026 | 0.069 | 0.875 | 0.009 | 0.796 | |
| 9 | D-Mannose | Fructose and mannose metabolism | 0.016 | 0.010 | 0.11 | 0.847 | 0.016 | 0.606 | |
| 10 | Glyceraldehyde | 0.021 | 0.026 | 0.044* | 0.833 | 0.021 | 0.606 | ||
| 11 | D-Quinovose | 0.001 | 0.010 | 0.052 | 0.958 | 0.001 | 0.197 | ||
| 12 | L-Fucose | Fructose and mannose metabolism | 0.002 | 0.010 | 0.055 | 0.944 | 0.002 | 0.197 | |
| 13 | L-Aspartate | Histidine metabolism | 0.001 | 0.010 | 0.981 | 0.986 | 0.001 | 0.121 | |
| 14 | L-Glutamate | Histidine metabolism | 0.004 | 0.022 | 0.103 | 0.917 | 0.004 | 0.302 | |
| 15 | L-Histidine | Histidine metabolism | 0.012 | 0.015 | 0.067 | 0.861 | 0.012 | 1 | 0.02 |
| 16 | N-Formylmethionine | 0.021 | 0.015 | 0.031* | 0.833 | 0.021 | 0.439 | ||
| 17 | Arg–Ala | 0.043 | 0.026 | 0.101 | 0.792 | 0.043 | 0.302 | ||
| 18 | His–Glu | 0.016 | 0.015 | 0.054 | 0.847 | 0.016 | 1 | ||
| 19 | Hypoxanthine | Purine metabolism | 0.001 | 0.016 | 0.977 | 1 | 0.001 | 0.121 | |
| 20 | Xanthine | Purine metabolism | 0.004 | 0.010 | 0.134 | 0.917 | 0.004 | 0.944 | 0.039 |
| 21 | L-Gulonic gamma-lactone | Ascorbate and aldarate metabolism | 0.004 | 0.015 | 0.112 | 0.917 | 0.004 | 0.071 | |
| 22 | N1-Methyl-2-pyridone-5-carboxamide | Nicotinate and nicotinamide (NAM) metabolism | 0.001 | 0.009 | 0.983 | 1 | 0.001 | 1 | |
| 23 | Phenol | 0.083 | 0.044 | 0.239 | 0.083 | 0.796 | |||
| 24 | Bilirubin | Porphyrin and chlorophyll metabolism | 0.068 | 0.047 | 0.098 | 0.068 | 0.439 | ||
Shown are the results of statistical analysis in normalized metabolite levels in PARK14 cases and matched controls, case-control comparison by the Mann–Whitney U-test, t-test after FDR (false discovery rate) correction, binary logistic regression (with age at exam and gender as covariates), and receiver operator area under the curve (ROC AUC) assessments of predictive ability in diagnosing PLA2G6 mutations and advanced stages (H&Y ≥ 3).
*p < 0.05.