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. 2022 Aug 19;11(16):2580. doi: 10.3390/cells11162580

Figure 2.

Figure 2

Setdb1 regulates osteoblast proliferation and bone formation. (A) The protein level of Setdb1 and PCNA in MC3T3-E1 cells by Western blotting after transfection of si-Setdb1, pcDNA3.1-Setdb1, or the corresponding controls (n = 3). (B) Cell proliferation evaluated by CCK-8 assays with the changes of Setdb1 (n = 3). Repeated measures ANOVA. ** p < 0.01 vs. the cells transfected with si-NC. ## p < 0.01 vs. the cells transfected with pcDNA3.1. (C) The EdU labeling assays in MC3T3-E1 cells from each group (n = 3). Scale bar, 50 μm. (D) Representative images of micro-CT and three-dimension reconstruction for the distal femurs of mice in the indicated groups (n =  5). (E) The three-dimensional measurement of the bone mineral density (BMD), trabecular thickness (Tb. Th), the ratio of bone volume to total volume (BV/TV), and trabecular number (Tb. N) in the ROI region of the distal femurs of mice from each group (n =  5). One-way ANOVA followed by LSD’s multiple comparisons test. ** p < 0.01 vs. the mice injected with (DSS)6-liposome-si-NC. (F) H&E staining showing trabecular microarchitecture of the distal femurs from each group (n =  5). Scale bar, 500 μm. (G) Goldner’s trichrome staining showing newly formed bone in distal femurs of mice in the indicated groups (n =  5). Scale bar, 50 μm. (H) The load-deflection curves for the respective groups (n = 5). (I) The biomechanical parameters of femurs in the indicated groups (n =  5). One-way ANOVA followed by LSD’s multiple comparisons test. ** p < 0.01 vs. the mice injected with (DSS)6-liposome-si-NC.