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. 2022 Aug 21;11(16):2606. doi: 10.3390/cells11162606

Figure 4.

Figure 4

Microarray analysis of mouse small intestinal organoids stimulated with IFN-γ, IL-1β and TNF-α. (A) Heat maps showing the top-30 up- and downregulated genes upon stimulation of organoids with cytokines. (B) Expression levels (log2) of Muc2, Ocln, Reg3g, Tff3, Cldn2 and Cldn3 in organoids after stimulation with vehicle or the cytokines IFN-γ, IL-1β and TNF-α (10 ng/mL). n = 6. (C) Gene set enrichment analyses (GSEA) for tight junctions after IFN-y treatment (left) and antimicrobial peptides after TNF-α treatment (right) with normalized enrichment scores (NES) of 1.96 and 2.04, respectively. The Y-axis shows the enrichment score, while the X-axis represents the specific gene ontology (tight junction or antimicrobial peptide) set genes. The green line connects the points of the enrichment score and genes. Normalization was conducted using MAS 5.0. Analysis of microarray experiments conducted performed using RStudio software (v1.4). Comparative analysis was conducted using the limma R package (v3.46.0). Moderated t statistic was used for statistical analysis.