Figure A3.

MaR1 protects against ferroptosis-mediated impairment of osteogenic differentiation of osteoblasts by modulating NRF2 signaling in T2DOP. (A) After seven days of osteogenic induction, the protein levels of COLI, ALP, and RUNX2 were determined using Western blot analysis. (B) Quantification of gray-value expression in the Western blotting. (C) ALP staining after seven days of osteogenic induction. Scale bar = 200 μm. Data are presented as the means ± SDs from three separate experiments (n = 3). ns p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. SiNC: siRNA negative control; siNRF2: siRNA of NRF2.