Figure 9.

Mechanistic analyses of the role of H19 in regulating TGF-β2-induced EMT in lens epithelial cells. (a–d) SRA01/04 cells were first infected with H19-overexpressing lentiviruses or negative control for at least 24 h and further treated with TGF-β2 (5 ng/mL, 24 h). Protein levels of p-Smad2, p-Smad3, total Smad2/3, and Smad4 were examined, using GAPDH as an internal control. Representative western blots and densitometric analyses (mean ± SD) are shown (n = 3 per group). * p < 0.05, ** p < 0.01, *** p < 0.001.