Figure 2.

Transcriptome analysis of monocytes from the livers of mice infected with E. histolytica or L. monocytogenes. (A) Gating and mRNA purification strategy for liver-specific Ly6C^hi and Ly6C^lo monocytes from E. histolytica- and L. monocytogenes-infected mice. (B) VENN diagram of transcriptome analysis of Ly6C^hi and Ly6C^lo monocytes shows significantly regulated genes of Ly6C^hi and Ly6C^lo monocytes from both infection models. PANTHER GO-slim analysis was performed to identify biological pathways involving significantly regulated genes in both monocyte populations. (C) Principal component analysis of transcriptomic data from Ly6C^hi and Ly6C^lo monocytes from naïve and E. histolytica- and L. monocytogenes-infected mice.