Figure 4.

Marked differences in expression of surface markers by Ly6C^hi monocytes after infection with E. histolytica or L. monocytogenes (A) Heatmap depicting differential expression of mRNA encoding selected markers on the surface of liver Ly6C^hi monocytes after infection with E. histolytica (E. his) or L. monocytogenes (L. m). (B) Normalized mRNA counts of selected surface marker genes (from transcriptome analysis). (C) Percentage of CD14⁺, CD38⁺, CD74⁺, and CD86⁺ Ly6C^hi monocytes during the course of infection at the indicated time points post-infection (measured by flow cytometry). (D) Histogram and MFI of CD14⁺, CD38⁺, CD74⁺, and CD86⁺ Ly6C^hi monocytes during the course of infection. Data in C were pooled from three independent experiments. Data in D are representative of one of these three experiments and all data are presented as the mean ± SEM (* p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001; Mann-Whitney U test).