Figure 5.

New Ly6C^hi monocyte subsets identified according to expression of selected surface markers and IRF2. (A) Gating strategy, histogram, and percentage of IRF2⁺ Ly6C^hi monocytes from livers of naïve mice and from E. histolytica- and L. monocytogenes-infected mice on day 3 post infecion. (B) Gating strategy (exemplary for CD14⁺ gated cells) based on the FMO control to determine CD14⁺, CD38⁺, CD74⁺, and CD86⁺ monocytes within the IRF2⁺ Ly6C^hi monocyte population from naïve mice and from both infection models on day 3 post infection. (C) UMAP plots of Ly6C^hi monocytes (including CD14⁺, CD86⁺, CD74⁺, CD38⁺) from naïve mice and from E. histolytica- and L. monocytogenes-infected mice (down sampled to 90,000 cells per sample). Six samples per source material were included in the analysis. (D) Cluster heatmap table showing surface marker and IRF2 expression by Ly6C^hi monocytes, cluster events, and integration of cluster events in the UMAP plot (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; Mann-Whitney U test).