Table 1.

PCR conditions for amplification of different genes.

Gene	Denaturation	Annealing	Elongation
β-actin	94 °C for 3 min	56.7 °C for 45 s	72 °C for 1 min in 30 cycles
ACSL1	94 °C for 3 min	61 °C for 45 s	72 °C for 1 min in 30 cycles
C/EBPα	94 °C for 3 min	61 °C for 45 s	72 °C for 1 min in 30 cycles
PPARγ	94 °C for 3 min	61 °C for 45 s	72 °C for 1 min in 30 cycles
SREBP-1c	94 °C for 3 min	61 °C for 45 s	72 °C for 1 min in 30 cycles