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Comparison of amino acid sequences of the Met1-to-Asp244 regions of various EnvZ proteins. Invariant residues in the N-terminal region (Met1 to Ile179) are indicated by stars beneath the sequence. The I box is enclosed in a box. The TM1 and TM2 domains and the sequence containing the H243 site of phosphorylation (H box) are underlined. Amino acid substitutions of mutants isolated in this study are indicated by arrowheads above the sequence.
