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Outer membrane protein analysis. AT142pcnB cells containing various envZ alleles were grown on MacConkey medium, and outer membrane proteins were prepared and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis as described previously (5). Lane 1, pJW49 (wild type); lane 2, pJW32P; lane 3, pJW35P; lane 4, pJW43P; lane 5, pJW48E. C, OmpC; F, OmpF; A, OmpA.
