Figure 3.

DNA damage induced by the hydroethanolic extract of C. album leaves (ECAL). (A) TUNEL staining for apoptosis in MDA-MB-231 cells incubated for 24 h. (A1) with the increasing concentrations of ECAL (1.7–1730 μg/mL); (A2) with DNAse (3000 U/mL); (A3) without treatment (control). Blue nuclear stain and green TUNEL stain. TUNEL-positive apoptotic cells (arrows) are those in which the green and blue colocalize to the same cell. Scale bar = 100 μM; (B) immunoblot images of proteins γH2AX, PARP1 and Caspase-3 cleaved (histone H3, as the internal control) wherein the fold changes have been presented below each panel.