Fig. 6.

Proximity-dependent biotin identification reveals proteins that reside near PfBLEB during gametocytogenesis. (A) Dot plots showing the ratio of the sum intensity in the presence/absence of biotin of proteins detected via mass spectrometry of PfBLEB-TurboID gametocytes. Thresholds were set to require more unique peptides than streptavidin. Sum intensity set to 1 for proteins absent in no biotin control. (B) An area-proportional Venn diagram comparing the proteins identified in both gametocyte TurboID replicates. (C and D) Immunofluorescence demonstrates that PF3D7_1435600-smV5 (red) localizes to distinct, punctate foci throughout schizogony (C) and gametocytogenesis (D), adjacent to basal complex protein PfMORN1 (green) or inner membrane complex-associated protein PfGAP45 (cyan). For (C), Airyscan images shown are single z slices for individual channels or maximum intensity projections of merged channels. For (D), the images shown are maximum intensity projection merged without and with brightfield image. (Scale bars, 1 µm.)