FIG. 3.

Runout kinetics after inhibition of initiation of replication and cell division. Cultures of strain BBC119 were grown under conditions of balanced growth in AB-glucose-Casamino-Acids medium at 14 and 37°C (see Table 1 for generation times). Rifampin and cephalexin were added at time zero, and incubation was continued at the growth temperature (A and B), while half of the 14°C culture was shifted to 37°C (C). Samples were taken at the indicated times and fixed, stained, and analyzed by flow cytometry. All distributions were normalized to contain the same number of cells.