FIG. 5.

Growth and dnaA gene expression after temperature upshift. Strain BBC119 was grown under conditions of balanced growth in AB-glucose-Casamino Acids medium at 14°C, and part of the culture was shifted to 37°C at the time indicated by the arrow. (A) Growth was followed by measurement of OD₄₅₀ and the culture was diluted when the OD₄₅₀ reached 0.5. Samples were taken for measurement of DnaA–β-galactosidase specific activity. (B) Differential plot of values for DnaA–β-galactosidase activity against OD₄₅₀ from panel A. Open symbols: 14°C; black symbols, 37°C.