TABLE 1.

Cell cycle parameters at different growth temperatures^a

Growth temp (°C)	No. of origins/ cell	DNA (g.e.)/cell	τ (min)	C/τb	D/τc	C (min)	D (min)
37	7.2	5.0	27.5	1.53	1.49	42	41
30	7.2	5.2	43	1.40	1.56	60	67
21	6.5	3.6	120	1.35	1.22	160	145
14	6.5	3.2	465	1.39	0.89	645	415

^aBBC119 was grown under conditions of balanced growth in AB-thiamine-glucose-Casamino Acids medium at the indicated temperatures. Samples were taken for flow cytometric analysis of DNA and origins. 

^bDetermined from the Southern blot analysis of origins per termini (O/T), using the formula O/T = 2^C/τ. 

^cDetermined from the formula genome equivalents (g.e.)/cell = [(O/T − 1)/lnO/T] · 2^D/τ (8).