Fig. 5.

Subclustering of DN1ps indicates their function in controlling evening activity. (A) Clustering of DN1ps using key molecules expressed in these cells. (B) trans-Tango experiment of the Vglut-DBD, per-AD split-GAL4 driver line stained with anti-GFP (trans-Tango positive neurons), anti-PER, and anti-RFP (GAL4- expressing neurons). Several neurons in the dorsal and lateral part of the brain are downstream of the Vglut-positive DN1 neurons. Notably, all LNds are included, indicating an anatomical connection of siesta behavior with cells controlling evening activity. For details see discussion.