| Advantages |
Disadvantages |
Advantages |
Disadvantages |
| Simple |
Does not mimic in vivo structure |
More like in vivo structure |
Expensive |
| Reproducible |
Fewer interactions with environment |
Niches are available |
Time consuming |
| Inexpensive |
Access to unlimited amount of nutrients from media |
Access to nutrients is not unlimited, varies |
Less reproducible |
| Less diverse phenotype and polarity |
Can form organs or spheroid clusters of cells |
More complex and difficult to carry out |
| Altered cell morphology |
Allows study of cell-cell and cell-ECM interactions |
Fewer interactions with environment |
