FIG. 4.

Cross-linking identifies interactions of TraC with the pKM101 transfer machinery. Shown are Western blot analyses with TraC-specific antiserum after SDS-PAGE of total cell lysates of FM433 (lane −) and FM433 carrying pKM101 (lane 101) or derivatives with transposon insertions in traM, traB, traC, eex, traD, or traE (lanes B, C, Ex, D, and E, respectively) (A), total cell lysates of the same strains after cross-linking with BS³ (B), and cell lysates and cross-linked samples of FM433 and FM433/pTrcTraC (C). Arrows indicate higher-molecular-weight complexes formed after cross-linking of TraC.