FIG. 5.

TraC associates with an extracellular macromolecular structure in FM433/pKM101. Macromolecular surface structures were isolated from FM433 carrying pKM101 and mutant derivatives. Cells were grown on LB agar plates and subjected to shearing, and the resulting samples were analyzed by SDS-PAGE and Western blotting with TraC-specific antiserum. (A) C, Total cell lysates; S1, supernatants after shearing; S2, supernatants after high-speed centrifugation; P, pellets after high-speed centrifugation. TraC detected in the pellet fractions is indicated by arrows. Lanes are labeled as in Fig. 4. (B) Analysis for content of periplasmic and cytoplasmic proteins with MalE- and SelA-specific antisera.