FIG. 6.

Analysis of TraC-containing high-molecular-weight structures by gel filtration chromatography. Surface structures isolated from FM433/pKM101 (A) and FM433 (B) were subjected to gel filtration on a Superose 6 column. Shown is analysis of column fractions by SDS-PAGE followed by Western blotting with specific antiserum; the arrow indicates TraC in a high-molecular-weight complex. Molecular masses of reference proteins for calibration of the gel filtration column: F, ferritin (440 kDa); B, bovine serum albumin (68 kDa); C, cytochrome c (12 kDa).