TABLE 2.

Compensation of TraC deficiency by extracellular complementation

Donor, FM433 carrying:	Helper, FM433 carrying:	Recipient	Transconjugants/ donor · ha
pKM101		WL400	1.1 × 10−1
pKM101traC::Tn5		WL400	≤10−10
pKM101traC::Tn5	pGW2137	WL400	3.7 × 10−8
pKM101traC::Tn5	pACYC184	WL400	≤10−10
pKM101traC::Tn5	pKM101traM::TnphoA	WL400	≤10−10
pKM101traC::Tn5	pKM101traD::Tn5	WL400	≤10−10
pKM101traC::Tn5	pTrc200	WL400	≤10−10
pKM101traC::Tn5	pTrcTraC	WL400	≤10−10
pKM101traC::Tn5		WL400 pTrc200	≤10−10
pKM101traC::Tn5		WL400 pTrcTraC	≤10−10

^aResults from two to five independent experiments.