TABLE 1.
Bacterial strains and plasmids used in this study
| Strain (alternate name) or plasmid | Genotype or characteristics | Reference or source |
|---|---|---|
| H. pylori | ||
| 26695 (88-21) | Wild type | 48 |
| 84-183 | Wild type | 50 |
| HPK5 (98-716) | Wild type | 49 |
| 98-712 | 84-183, StrrureB::aphA | This study |
| 98-706 | 84-183, Spcr | This study |
| 98-707 | HPK5, Strr, pHP1 (Kanr, Ampr) | This study |
| 98-708 | HPK1, Spcr | This study |
| 98-700 | 84-183/0333::aphA | This study |
| 98-701 | HPK5/0333::aphA | This study |
| 98-709 | HPK5/0333::aphA, pANDO1 (Ampr, Camr) | This study |
| C5 (98-710) | HPK5, Pure-HP0333-cat-′ureAB | This study |
| C5G9 (98-702) | HPK5/0333::aphA, Pure-HP0333-cat-′ureAB | This study |
| C5G10 (98-711) | HPK5, Pure-HP0333::aphA-cat-′ureAB | This study |
| E. coli DH5α | endA1 hsdR17 (rK− mK+) supE44 thi-1 recA gyrA (Nalr) relA1 Δ(argF-lacZYA)U169 deoR [φ80dlacΔ(lacZ)M15] | |
| Plasmids | ||
| pGEM-T Easy | ColE1, Ampr, PCR cloning vector | Promega |
| pDAI12 | pGEM-T Easy containing HP0333 | This study |
| pUK4K | ColE1 (Kanr, Ampr) | Pharmacia |
| pDPR2 | pDAI12, HP0333::aphA | This study |
| pHP1 | H. pylori-E. coli shuttle vector (Ampr, Kanr) | 31 |
| pUC19 | ColE1, Ampr | New England Biolabs |
| pBSC103 | Ampr, Camr | 54 |
| pANDO2 | pUC19, Pure-HP0333-cat-′ureAB | This study |