Fig. 4.

S408 pseudophosphorylation modifies intermolecular interactions between the free unstructured region and the α-helical bundle. (A) PRE analyses took advantage of C409 (Left) within occludin 383–412^S408A (blue) and 383–412^S408D (red). (B) Intermolecular interactions between each spin-labeled peptide and the occludin^413-522 α-helical bundle are shown. Residues L40, G411, and S412 derive from the occludin 413–522 N-terminal extension adding during synthesis and are labeled in gray. These were not affected by S408 pseudophosphorylation.