Fig. 7.
S408 phosphorylation may cause the unstructured region to mask the ZO-1 binding site within the occludin C-terminal α-helical bundle. (A) Structure of occludin 383–522S408A. The structure of the α-helical bundle is based on reported X-ray diffraction data (21). The position of the unstructured region (383–413) was generated in MUSTER software (45). (B) Structure of occludin 383–522S408D. Two views oriented at right angles to one another are shown for each structure. Colors (blue, red) indicate the electrostatic potential as determined via Poisson–Boltzmann equation.