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. 2022 Aug 15;119(34):e2206208119. doi: 10.1073/pnas.2206208119

Fig. 7.

Fig. 7.

GBM transcriptomes of the TCGA dataset are clustered according to TH17 and TREG cell signatures. (A) UMAP representation of the transcriptomes of the TCGA GBM cohort (n = 154). (B–D) GSVA was run on the variance-stabilized expression data of the TCGA cohort for the indicated gene sets. Resulting GSVA scores were normalized to the interval 0 to 1 and projected onto the UMAP representation. (B) Projection of our GBM CD4+ T cell signature (i.e., DEGs in GBM vs. normal-appearing brain CD4+ T cells). (C) Projection of the IL-6–JAK–STAT3 hallmark pathway signature. (D) Projection of a published TREG cell signature (GSE15659). Note that the GBM CD4+ TIL signature coincides with clusters 1 and 3. The IL-6–JAK–STAT3 signature projected on cluster 3, and the TREG cell signature projected on cluster 1 (and 4).