FIG. 1.

Southern hybridization of H. pseudoflava DNA. Southern hybridization was performed with random primed probes synthesized by using a 0.5-kb PCR product covering the CO-DH small subunit as a template. Shown are gels subjected to agarose gel electrophoresis (A) and Southern hybridization with random primed probes (B). H. pseudoflava chromosomal and plasmid DNAs were digested with HindIII (H), EcoRI (E), BamHI (B), SalI (S), and SmaI (M). λ DNA digested with HindIII (λ) was used as a size marker.