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Identification of transcriptional start site of cut genes. The transcriptional start of cut genes was identified by primer extension mapping using a 32P-labeled 30-mer oligonucleotide primer which is complementary to codons 7 to 16 of the HpsCut medium subunit. Extension products (lane P) were analyzed in parallel with the sequencing ladder (lanes G, A, T, and C) primed with the same primer. The asterisk shows the transcriptional start site.
