TABLE 1.
Bacterial strains, phages, and plasmids used in this study
| Bacterial strain, phage, or plasmid | Description | Source or reference |
|---|---|---|
| Bacterial strains | ||
| H. pseudoflava | Wild type (DSM 1084) | 43 |
| E. coli | ||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10(Tetr)] | Stratagene |
| XL1-Blue MRA (P2) | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 gyrA96 relA1 lac (P2 lysogen) | Stratagene |
| Phages | ||
| Lambda EMBL3 | BamHI-digested arms | Stratagene |
| Lambda clone 311 | Lambda EMBL3 clone containing cut genes of H. pseudoflava | This study |
| Plasmids | ||
| pUC18 | 2.69-kb cloning or sequencing vector; Ampr | 44 |
| pBKS2 | pUC18 containing 1.5-kb SalI fragment from clone 311 | This study |
| pBKX1 | pUC18 containing 3.5-kb SmaI fragment from clone 311 | This study |