Fig. 2.

Protein sequence alignment of selected nematode AVR-14B homologs showing ligand-binding loops, transmembrane domain (TM1-TM4), pre-TM1 region, and TM2-TM3 linker region. Residues of the amino acid agonist (AAA) motif in the loops that contact bound glutamate are illustrated in maroon. Residues that reduce agonist sensitivity are highlighted in purple in the pre-TM1 and TM2-TM3 linker region. PAR motif, which is characteristic of LGICs, is coded in orange in the TM2 region. The sites of mutation that reduced IVM sensitivity are illustrated in a black box in the TM regions. Cel, C. elegans; Bma, B. malayi; Hco, H. contortus; Dim, D. immitis; Con, C. oncophora.