Fig. 6.

IVM acts as an inhibitor of NA-mediated potentiation of L-glutamate–gated currents in the Xenopus oocytes expressing B. malayi AVR-14B. (A) Representative inward current responses showing the effect of IVM (0.1 nM IVM in top trace, n = 6; 1 pM in bottom trace; n = 6) on combination of NA with L- glutamate (L-glu; 30 µM). (B) Concentration-response curves showing the potent inhibitory effect of 0.1 nM IVM on combination of NA (0.1 to 0.3 µM) with L-glutamate (30 µM) when normalized to 30-mM L-glutamate current response. (C) Concentration-response curves showing effect of 1 pM IVM on combination of NA (0.1 to 3 µM) with L-glutamate (30 µM) when normalized to 30-mM L-glutamate current response. EC₅₀ values were 0.4 µM (pEC₅₀ = 6.4 ± 0.4) for NA and L-glutamate combination in the absence of IVM; 0.3 µM (pEC₅₀ = 6.5 ± 1.0) NA and L-glutamate combination in the presence of IVM. There was no significant difference between the Hill slopes. (D) Concentration-response curves showing effect of 1 pM IVM on combination of NA (0.1 to 3 µM) with L-glutamate (30 µM) when normalized to 30-mM L-glutamate AUC response. AUC (max) ± SEM, %, was 1,430.0 ± 168.3 for NA and L-glutamate combination in the absence of IVM; 295.5 ± 101.0 for NA and L-glutamate combination in the presence of IVM. L-glu, L-glutamate. Error bars in B and C represent SEM.