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. 2022 Aug 15;119(34):e2207841119. doi: 10.1073/pnas.2207841119

Fig. 2.

Fig. 2.

T cell response and protective effect after vaccination. (A) Timeline for vaccination and blood withdrawal. (B) Changes of cytokines and chemokines in the mice treated by blank or OVA mRNA-formulated LNPs for 24 h. (C) OVA-specific antibody titers in the mice treated by 113-O12B/mOVA and ALC-0315/mOVA on day 12. (D) Representative flow cytometry diagrams of IFN-γ-positive cells within CD3+ CD8+ T cells 7 d after second vaccination. (E) Time-dependent changes of IFN-γ-positive cells 7, 14, and 28 d after second vaccination. (F) Tumor volumes of B16F10-OVA tumor model. (G) Lungs collected 18 d after the intravenous injection of B16F10-OVA cells. UT: Untreteated. The error bar around each data point is the SD. Tukey's multiple comparisons test was used to calculate the statistical significance. *P < 0.05 was considered statistically significant. **P < 0.01 and ***P < 0.001 were considered highly significant.