Fig. 4.
Therapeutic effect on B16F10-OVA tumor after vaccination by mRNA cancer vaccine. (A) Representative flow cytometry diagrams and percentages of CD3+ CD8+ T cells bearing T cell receptors binding to H-2Kb OVA tetramer-SIINFEKL within PBMCs 7 d after the second vaccination. (B) ELISpot images and spot nos. of IFN-γ-secreting T cells within PBMCs of vaccinated mice. (C) Tumor volumes of B16F10-OVA model posttreatment. (D) Lungs collected 18 d after the intravenous injection of B16F10-OVA cells. The error bar around each data point is the SD. Tukey's multiple comparisons test was used to calculate the statistical significance. *P < 0.05 was considered statistically significant. **P < 0.01 and ***P < 0.001 were considered highly significant.