TABLE 2.
Kinetic parameters for PrpC citrate synthase and 2-methylcitrate synthase activity
| Parameter | Valuea |
|---|---|
| Citrate synthase activity | |
| Km acetyl-CoA | 285 ± 20 μM |
| Km oxaloacetate | 14 ± 4 μM |
| Vmax | 2 ± 0.1 μmol/min/mg |
| kcat | 1.4 ± 0.1b s−1 |
| kcat/Km acetyl-CoA | 5 × 103 M−1 s−1 |
| 2-Methylcitrate synthase activity | |
| Km propionyl-CoA | 48 ± 8 μM |
| Km oxaloacetate | 12 ± 2 μM |
| Vmax | 10 ± 0.3 μmol/min/mg |
| kcat | 7.4 ± 0.2b s−1 |
| kcat/Km propionyl-CoA | 150 × 103 M−1 s−1 |
a
Values reported as mean ± standard deviation.
b
kcat values were calculated per subunit of His-tagged PrpC.