Table 6.
Primer sequences and reaction conditions.
| Method | Gene | Primer Sequences | Product Size (bp) | Ta (°C) | Reference |
|---|---|---|---|---|---|
| MS-HRM | MIR-34A | F 5/-tttttttttaggtggaggagatgt-3/ R 5/-ccaaacaaacccaaacaaaac-3/ |
155 | 64 | [17] |
| MIR-34B/C | F 5/-ttgttattaaaataaggtatagtatta-3/ R 5/-cgcttctcaaacatcttctct-3/ |
99 | 56 | ||
| MS-PCR | MIR-203 | MF 5/-gagtattttcggtttagacgagac-3/ MR 5/-ccttttatacgacgcaaccg-3/ UMF 5/-tttgagtatttttggtttagatgagat-3/ UMR 5/-aacaccttttatacaacacaacca-3/ |
287 | 60 | [22] |
| MIR-129-2 | MF 5/-gagttgggggatcgcggac-3/ MR 5/-atataccgacttcttcgattcgccg-3/ |
189 | 62 | [16] | |
| UMF 5/-gagttgggggattgtggat-3/ UMR 5/-aatataccaacttcttcaattcacca-3/ |
188 | 60 |
Keys: MS-HRM—Methylation-Sensitive High-Resolution-Melting, MS-PCR—Methylation-specific polymerase chain reaction, F—forward primer, R—reverse primer, M—methylated allele, UM—unmethylated allele, Ta—annealing temperature, bp—basic pairs.