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Effects of the dctA::spc mutation on fumarate transport. Cultures were grown aerobically in L broth, harvested, washed in M9 salts solution, and then used to measure the rate of [2,3-14C]fumarate uptake, in duplicate, as described in Materials and Methods. The strains were AN387 (dctA+) (□), MDO800 (dctA::spc) (▵), AN387(pDctA) (■), and MDO800(pDctA) (▴). Standard deviations are shown.
