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Specific gene deletion and disruption of taG and taH by a kanamycin resistance gene insertion into pPY06 replacing a 2.3-kb BalI site, forming the plasmid pPY06B1-Kan (A), and a 44-bp fragment at the beginning of taG (at a specific StuI site formed by PCR), forming the plasmid pPY06St-Kan (B). The resulting plasmids were linearized and electroporated into M. xanthus ER-15. Restriction enzymes: St, StuI; X, XhoI; Bl, BalI; H2, HindII.
