TABLE 1.
Electron acceptors for ethylbenzene dehydrogenase and 1-phenylethanol dehydrogenase activities in cell extracts
| Electron acceptor tested | Activitya of ethylbenzene dehydrogenase (%) | Activitya of 1-phenylethanol dehydrogenase* (%) |
|---|---|---|
| p-Benzoquinone (E0′ = +293 mV) | 100 | 100 |
| Oxygen (E0′ = +820 mV) | 40 | 30 |
| Nitrate (E0′, nitrate/nitrite = +430 mV) | 11 | 17 |
| Dichlorophenol indophenol (E0′ = +217 mV) | 58 | 29 |
| 1,4-Napthoquinone (E0′ = +36 mV) | <1 | 22 |
| Methylene blue (E0′ = +11 mV) | 2 | 6 |
| Menaquinone (E0′ = −80 mV) | 6 | ND |
| NAD+ (E0′ = −320 mV) | NDb | 25 |
| NADP+ (E0′ = −320 mV) | ND | 10 |
| None | 10 | 10 |
a
Activity measured as percentage of activity in cell extracts with p-benzoquinone as the electron acceptor (approximately 10 nmol min−1 [mg of protein]−1 for ethylbenzene dehydrogenase and approximately 15 nmol min−1 [mg of protein]−1 for 1-phenylethanol dehydrogenase). Assay conditions were as described in Materials and Methods. All electron acceptors tested were at 1 mM in the in vitro assay (except for oxygen).
b
ND, not determined.