Figure 2.

Analysis of hTERT expression, stem cell markers and differentiation potential of hTERT transduced ADSC. (A) Expression of hTERT before and after hTERT transduction as revealed by Western Blot technique. (B) Flow cytometry analysis showing that the expression of surface markers CD73, CD90 and CD105, required for mesenchymal stromal/stem cells definition, was not lost after lentiviral transduction up to the 31st passage (n = 3). (C) Evaluation of multilineage differentiation capacity of hTERT transduced ADSC. Note that, a short time after transduction (5th passage), the cells were able to properly differentiate into osteogenic and adipogenic lineages, and less into chondrocytes, since the Alcian Blue staining was hardly noticed.